Vice President Mike Pence and wife Karen on Tuesday tested negative again for COVID-19 ahead of the Wednesday night vice presidential debate.

A Pence aide confirmed to The Post that the couple remain negative for the serious respiratory bug that infected President Trump and other top Republicans.

The Pences traveled Monday to Salt Lake City, where the veep will debate Democratic running mate Sen. Kamala Harris of California.

Mike Pence is “healthy, without any COVID-19 symptoms, and has continued to have daily COVID-19 antigen tests and intermittent PCR tests which have all resulted as negative,” his physician, Dr. Jesse Schonau, said in a public memo Tuesday afternoon.

Pence “is not in close contact with any of the individuals who have tested positive for COVID-19, including President Donald J. Trump and senior members of the White House administration, according to the guidelines of the Centers for Disease Control (CDC),” the doctor wrote.

Pence “is encouraged to go about his normal activities and does not need to quarantine,” according to Schonau.

Still, the vice president did not leave his official residence on Friday, Saturday or Sunday “out of an abundance of caution,” the doctor’s memo said.

Trump’s diagnosis Thursday followed the first presidential debate Tuesday in Cleveland, Ohio, against Democratic nominee Joe Biden.

The vice presidential debate features new restrictions to limit the potential spread of the virus. The candidates will be separated by Plexiglas and will be 12 feet apart, rather than the originally planned 7 feet.

Attendees at the University of Utah-hosted forum will be required to wear masks. And journalists attending the debate are compelled to get tested by 5 p.m. Tuesday, whereas the Cleveland debate featured on-site and day-of rapid tests.

Pence, 61, has tested negative daily after a Sept. 26 Rose Garden event attended by many people who later tested positive for the virus.

The vice president also attended a “Prayer March” with Pastor Greg Laurie of Riverside, Calif., who contracted the virus, before the Rose Garden presentation of Supreme Court nominee Amy Coney Barrett.

Utah Republican Sen. Mike Lee is among the Rose Garden attendees who tested positive. Republican National Committee Chairwoman Ronna Romney McDaniel, who was last near Trump the day before the Rose Garden event, also tested positive. She is a niece of Utah’s other senator, Republican Mitt Romney.

Trump campaign manager Bill Stepien, White House adviser Hope Hicks, former White House adviser Kellyanne Conway, Sen. Ron Johnson (R-Wis.), Sen. Thom Tillis (R-NC) and former New Jersey Gov. Chris Christie are among the prominent Republicans infected in the past week.

White House press secretary Kayleigh McEnany on Monday became the latest high-profile Trump aide to test positive.

Trump returned to the White House on Monday night after three days at the Walter Reed military hospital in Bethesda, Maryland. Trump tweeted Tuesday that he intends to debate Biden on Oct. 15 in Miami.

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